Cells of a triple-mutant yeast strain (leu- his- trp-) were spread either on minimal medium or on minimal medium supplemented with various combinations of histidine, leucine, or tryptophan. The cultures were grown at either 25C or 37C for 3 days. Colony numbers in each plate were counted, and the data are listed in the following table.
What kind of mutation most probably causes the his- phenotype?What kind of mutation most probably causes the leu- phenotype?
My questions : What is the meaning of confluent in this context ? Can anyone give me a hint to solve this question ? I have no clue how to solve this.