Is there any way of determining which X chromosome a female inherited from her father and which from her mom ?
$\begingroup$
$\endgroup$
-
1$\begingroup$ You could try to follow this up by sequencing the X chromosome from the father and try to find unique polymorphisms which are not present in the X chromosome of the mother. You can than try to find them in the childs "X". This makes not much sense though, since one of the X chromosomes is ramdomly inactivated. $\endgroup$ – Chris♦ Jan 25 '14 at 11:11
-
$\begingroup$ @Chris So, we could use RFLP for this ? Could we also use FISH ? $\endgroup$ – biogirl Jan 25 '14 at 11:24
-
1$\begingroup$ RFLP could work, I am not sure if the resolution of fish is good enough. $\endgroup$ – Chris♦ Jan 25 '14 at 11:38
-
$\begingroup$ @Chris Thanks! I don't know much about FISH, so what exactly do you mean when you say that resolution won't be good ? $\endgroup$ – biogirl Jan 25 '14 at 11:41
-
3$\begingroup$ For FISH you need a probe that binds to the chromosome. If the difference you want to see is so small (a SNP for example), that the probe will bind anyway, than the resolution of the method is too small to detect the difference. Insertions or deletions are bigger an can be detected. $\endgroup$ – Chris♦ Jan 25 '14 at 11:48
$\begingroup$
$\endgroup$
yes it is possible... in placenta (trophectoderm) the paternal X is epigenetically silenced [ref].
That is if you do the test in the pre/neo-natal stage. Otherwise, at later stages, you have to check by DNA fingerprinting + karyotyping/FISH as Chris suggested.
