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Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells.

The caveat is that I would like to do so without fixing the cells or waiting for them to adhere to the filter. The small molecules are small enough to diffuse through the filter.

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  • $\begingroup$ perhaps you can dip the filter plate in a medium containing the small molecules.. and then keep changing the bath after few days.. btw you want a batch or continuous system?? $\endgroup$
    – WYSIWYG
    Jun 10, 2013 at 7:55
  • $\begingroup$ @WYSIWYG, Batch should be fine. The question is what would be the setup to do so. Would it be as simple as reversing a 96 well lid over and placing the filter plate on top? What about the wetting? $\endgroup$
    – bobthejoe
    Jun 10, 2013 at 22:46
  • $\begingroup$ I am not very sure but you may try the plates used for growing ES cells over feeder layers separated by a membrane $\endgroup$
    – WYSIWYG
    Jun 11, 2013 at 4:38

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Perhaps you're looking for these hanging inserts, for example: "Millicells"

example of inserts

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  • $\begingroup$ Can you elaborate a bit more on why this meets the OP's requirements? $\endgroup$
    – jonsca
    Nov 2, 2014 at 8:06
  • $\begingroup$ The original poster would like to feed his immobilized cells small molecules, without fixing or waiting for adherence. The inserts contain a membrane, which forms a permeable barrier, to allow diffusion of small molecules, and site for attachment. The insert itself is free to move and hang in different culture media/wells, so no need to trypsinize or resolubilize them, and provides containment of the non-attached cells. $\endgroup$
    – Royco
    Nov 3, 2014 at 10:40

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