Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells.

The caveat is that I would like to do so without fixing the cells or waiting for them to adhere to the filter. The small molecules are small enough to diffuse through the filter.

  • $\begingroup$ perhaps you can dip the filter plate in a medium containing the small molecules.. and then keep changing the bath after few days.. btw you want a batch or continuous system?? $\endgroup$
    Jun 10 '13 at 7:55
  • $\begingroup$ @WYSIWYG, Batch should be fine. The question is what would be the setup to do so. Would it be as simple as reversing a 96 well lid over and placing the filter plate on top? What about the wetting? $\endgroup$
    – bobthejoe
    Jun 10 '13 at 22:46
  • $\begingroup$ I am not very sure but you may try the plates used for growing ES cells over feeder layers separated by a membrane $\endgroup$
    Jun 11 '13 at 4:38

Perhaps you're looking for these hanging inserts, for example: "Millicells"

example of inserts

  • $\begingroup$ Can you elaborate a bit more on why this meets the OP's requirements? $\endgroup$
    – jonsca
    Nov 2 '14 at 8:06
  • $\begingroup$ The original poster would like to feed his immobilized cells small molecules, without fixing or waiting for adherence. The inserts contain a membrane, which forms a permeable barrier, to allow diffusion of small molecules, and site for attachment. The insert itself is free to move and hang in different culture media/wells, so no need to trypsinize or resolubilize them, and provides containment of the non-attached cells. $\endgroup$
    – Royco
    Nov 3 '14 at 10:40

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.