I am interested in using forskolin in cell culture medium. Does anyone know how to make solution of 10 microM forskolin in 5% ethanol or less. I would like to avoid using DMSO as a solvent. Thank you. L.
Sigma gives a solubility of 200 microMole in water containing 2% ethanol. They state:
Forskolin is soluble in water (with 2% ethanol) up to 0.2 mM by first dissolving in ethanol at 5 mg/mL and doing subsequent dilutions with water.
The datasheet can be found here.