I get air bubbles while pipetting small volumes. How can I avoid them ?

  • $\begingroup$ You can try to be slow? $\endgroup$ – golgicik Feb 28 '14 at 14:27
  • $\begingroup$ You have to pipet up slow and make sure your tip is (and stays) completely submerged in the solution. On the other hand it shouldn't be put in too far. $\endgroup$ – Chris Feb 28 '14 at 14:34

You're still pipetting too fast, you should move the piston slowly and evenly to avoid air bubbles. Also make sure that you wait a second or two for the liquid to rise before moving the tip out of the liquid reservoir.

Pipettes can also behave rather weird with any liquid that differs significantly in any physical property from water, e.g. very viscous solutions.

You can find a few more tips in this document from Eppendorf or this one from Thermo Scientific.

  • $\begingroup$ Sometimes with very viscous liquids (such as detergent solutions), it may be necessary to slowly "rinse" the inside of the pipette by doing a full pieptte up and down of the liquid before an accurate amount will be drawn. $\endgroup$ – user560 Mar 3 '14 at 23:51

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