Would expression of GroEL and GroES in erythrocytes be a potentially effective therapeutic strategy for sickle cell disease? Why or why not?
- GroEL and GroES are multiprotein chaperonin complexes: Not so easy to ectopically express
- Erythrocytes do not have nucleus: you cannot stably express anything in mature erythrocytes.
- If your aim is to do a stable gene expression (which would require genomic integration), the better way is to do a gene therapy: culture haematopoetic stem cells, Correct the mutation by homologous recombination. Put the cells back into the bone marrow.