Anyone have a short & sweet protocol for PCR amplifying a region of human DNA (chromosomal or mt, I don't care) extracted from a buccal sample: including validated primer sequences and preferred method of DNA isolation from a buccal swab. I'm looking for a new exercise to run in my classroom. We would be able to follow the amplification up with Sanger sequencing, done in-house.

  • $\begingroup$ Which possibilities do you have for the extraction? Would this be more "lab-like" or more "DIY-bio"-style? $\endgroup$
    – Chris
    Aug 5 '14 at 13:32

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