GC-content refers to the frequency of base pairs that are either C or G in the genome, or in other words the number of GC base pairs divided by the addition of the number of GC base pairs plus the number of AT base pairs.

$$GCcontent = \frac{N_{GC}}{N_{AT}+N_{GC}}$$


How does the GC-content evolve and why does the GC-content differ between populations/species/lineages? Does it evolve under Genetic drift only? Under selection? Intuitively, I'd say that the ratio of the probabilities of mutating from A or T to G or C should be an important factor driving the evolution of the GC-content. Does it? Does the overall mutation rate influence the GC-content? What other traits/forces influence the evolution of the GC-content?

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    $\begingroup$ GC base pairs are more stable than AU/AT base pairs. I could imagine situations in which this plays a role, though I don't know if this is actually a relevant effect. $\endgroup$ – Mad Scientist Apr 9 '14 at 18:22
  • $\begingroup$ Yes it is probably an important factor. Note that if this difference in stability is the only thing that explain the evolution of GC-content, then I'd expect that very little variation would exist between species and the equilibrium point should be given by $$\frac{P_{CG \space |\space AT}}{P_{CG\space |\space AT}+P_{AT\space |\space CG}}$$, where $P_{CG\space |\space AT}$ is the mutation rate from $A$ or $T$ to $C$ or $G$. $\endgroup$ – Remi.b Apr 11 '14 at 6:48

I think the key work here is 'evolve'. Overall GC/AT ratios change by mutations, whose rate is constant. The probability that given a mutation event that one base will be substituted by another one has been modeled in several ways where the probabilities of different mutations may or may not be the same.

Overall the GC content will tend to close to 50%. What causes GC rich genomes to become GC rich (60-70%) are that mutations to GC base pairs have selective advantages either in regions or in the genome overall that cause them to be retained. The mutation rate may be no different (or even lower) in GC rich organism (many of them are deep underground or deep underwater. GC rich genomes occur because AT->GC mutations convey an advantage and they stick around.

The reasons that the GC content migrates away from 50% fall into two categories I will call entropic and selective.

By entropic I mean specifically that coding sequences for genes and other features such as binding sites on the DNA or other features such as centromeres, which will cause the overall ratio to vary from 1 because the sequence is constrained by the information it contains. While coding regions have a ratio higher than 1, GC content tends to hover around 54%. Eukaryotes have GC islands and etc, but this also does not overall change the GC content

So gene rich genomes and typical functional features of the genome do not really explain some of the spectacularly high GC contents found; up to the 70% range. While the link above looks at GC bias in coding regions, its a given that any part of the genome that is merely a spacer between elements with specific functions will freely vary to GC if it is useful.

Selective factors for high GC content include high pressure and temperature environments for instance, which usually strongly bias to high GC content by this mechanism. You can imagine how this works: high GC content genomes are thermodynamically more stable and can survive the extra molecular collisions of higher energy of those environments more readily.

GC rich genomes are not simple adaptations to live with. All the genes for DNA oriented processes such as transcription, chromosome packing, DNA polymerase have to adjust a lot. As the organism adapts to hotter temperatures or higher pressures, each individual protein produced will have to also change to be stable and function in the new conditions. As such, these changes happen only over long evolutionary times. This is probably a good part of the reason why the archaea niches have not been superseded by eubacteria in all 1+ billion years since life has been on Earth.

  • $\begingroup$ Thanks for your answer. I am not sure I understand though. Let me try to rephrase differently and you'll correct me.Temperature or any factor that modifies the state_to_state specific mutation rate will modify the GC-content influence the GC-content and those factors are likely to vary from species to species explaining the differences observed. $\endgroup$ – Remi.b Apr 14 '14 at 7:37
  • $\begingroup$ Also the kind of sequence we consider (and the amount of DNA sequence that are of this type in a given genome) influence the GC-content. Coding sequences for example are not free to vary their information too much (usually it is only the third base pair that is free to vary). Did you say anything else? Did I understand? $\endgroup$ – Remi.b Apr 14 '14 at 7:38
  • $\begingroup$ I tweaked my answer. in short though, the mutation rate may be no different for these organisms, coding sequences can migrate to 70% GC. its all selection in certain environments. lmk if i'm making a good case here. $\endgroup$ – shigeta Apr 14 '14 at 14:27
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    $\begingroup$ Everything is clear now! I got what I missunderstood ;) Thanks a lot. $\endgroup$ – Remi.b Apr 14 '14 at 15:17
  • $\begingroup$ Glad to hear it! There is now a lot of research going on to identify processes which mediated and accelerate adaptation and therefore evolution. On this level there isn't so much controversy I think... $\endgroup$ – shigeta Apr 14 '14 at 17:31

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