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When performing a standard curve for some new primers to test for fold change, is it necessary to run the standard curve? I mean, in case your experiment has several genotypes for the same ecotype background, with correspondent control and treatments, must I run the standard curves for all this scenarios, or rather may I pick, just to say a random genotype of treated ones, see that it R=0.99 is or higher and efficiency 2? It is in order to not to waste material.

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Do the standard curve once for each primer pair to determine efficiencies. You can use the efficiency values for any number of replicates with any template.

I think we have somewhat discussed this issue in your previous post.

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  • $\begingroup$ sorry to disturb but then if i get an efficiency value using control tissue, this values may be used for treatments, isn´t it? $\endgroup$ – Katz May 12 '14 at 16:11
  • $\begingroup$ yep.. the efficiency is just the property of the primer and its template DNA.. $\endgroup$ – WYSIWYG May 12 '14 at 21:20

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