Is there any academic reference that shows α-tubulin is around 50-55 kDa? The only thing I found is some data sheets from companies. I need the real reference.
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1$\begingroup$ The data sheet is a good enough reference. They must have showed a gel picture also. If not then find details of the protein in Uniprot. $\endgroup$– WYSIWYGJun 10, 2014 at 6:48
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2 Answers
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This is not so easy to answer, since alpha-tubulin has at 10 known genes in this family (data from Ensembl):
- TUBA1A tubulin, alpha 1a
- TUBA1B tubulin, alpha 1b
- TUBA3E tubulin, alpha 3e
- TUBA3D tubulin, alpha 3d
- TUBA4A tubulin, alpha 4a
- TUBAL3 tubulin, alpha-like 3
- TUBA1C tubulin, alpha 1c
- TUBA3C tubulin, alpha 3c
- TUBA8 tubulin, alpha 8
- TUBAL3 tubulin, alpha-like 3
You can go on the site linked above and choose any of the proteins. I checked TUBA1A, and get a length of 451 amino acids and a weight of approximately 50,1 kDa.
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The paper titled Identification of α-tubulin as a granzyme B substrate during CTL-mediated apoptosis mentions it as 51KDa and the paper titled Carboxy-terminal amino acid sequence of α-tubulin from porcine brain mentions it as 55,000 Da. Hope that suffices.
answered Jun 10, 2014 at 6:48