Does anybody have any estimate on the number of RNA pol II molecules in the nucleus of a given human cell? For example, how many RNA polymerase type II protein complexes would there be on average at a given time in, say, a human immortalized lymphoblastoid cell line (LCL)?


it is highly variable even in a single cell type.

Dont know for a human cell but this is the range for ecoli: 1500-11400

check this site out. got this number from there (its quite useful for questions like these):


You can estimate protein copy numbers and there are techniques available for doing so. One of such techniques:


You can also calculate concentration using traditional techniques like western blot and elisa and calculate approximate copy-numbers (you have to also calculate average cell volume and number of cells for protein extraction.


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.