Assume that there are two different signals occurring on the ECG - during depolarization and repolarization in the standard ECG. I was advised not to use the standard ECG in measuring the exact electrical events during:

  • stages 0-2
  • 3-4

We can get data about the electric activity of neurons in vivo in Fig. 1 where some data about the electric activity of neurons is present. So my thought is that a similar technique could probably work with cardiomyocytes.

Fig. 1 Example of measurement for electric activity of neurons in vivo

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I estimate that there is about 18% uncertainty when using the standard ECG because of the peak in the stage 1 (Gibbs phenomenon) and those two complete different phases.

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    $\begingroup$ This is probably not possible in vivo, but there are various methods (voltage clamping, channel blockers) that you can use in vitro with individual cells and perhaps correlate the results with the aggregate activity of a larger piece of heart muscle. $\endgroup$ – jonsca Jul 16 '14 at 22:56
  • $\begingroup$ @jonsca I found that we can get data about the electric activity of neurons in vivo. So probably the same technique has been used with cardiomyocytes. $\endgroup$ – Léo Léopold Hertz 준영 Jul 17 '14 at 8:34
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    $\begingroup$ @Masi Neurons are easier because they aren't moving that much. $\endgroup$ – Nick Alexander Aug 11 '16 at 17:38
  • $\begingroup$ @NickSandor Can you please make your comment an answer and support it with evidence and/or sources. It is very interesting, but true. Can you say how much neurons and cardiomyocytes move, please. - - I am really interested in on any estimates about the topic because I have overlooked it for long time. - - Can you please state what you expect to be seen in cardiomyocyte activity? What is their metric in time-frequency plane or time-potential plane? I get mostly cloudy areas but different from neuron areas when studying spectrograms and other distributions. $\endgroup$ – Léo Léopold Hertz 준영 Aug 11 '16 at 18:08
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    $\begingroup$ I searched Pubmed for cardiomyocyte+"patch clamp", and read the first ~10 abstracts. Everyone measures plated cells. At best, these cells came from from hearts freshly minced in collagenase / EDTA. See PMIDs 27364017, 27076034, 26142302, 26241168, 26378152, to mention the most recent search results. $\endgroup$ – Nick Alexander Aug 11 '16 at 18:25

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