I found the link to a commercial product by Evrogen to normalise cDNA samples for gene discovery projects here:

The most up-to-date reference seems to be this Curr Protoc Mol Biol. paper by Bogdanova et al.: http://www.ncbi.nlm.nih.gov/pubmed/20373503

They claim their method is compatible with nextgen sequencing platforms:

cDNA normalization using duplex-specific nuclease (DSN) is a highly efficient approach that can be applied for normalization of full-length-enriched cDNA (Zhulidov et al., 2004; Zhulidov et al., 2005). The resulting cDNA contains equalized abundance of different transcripts and can be used for construction of cDNA libraries and for direct sequencing, including high-throughput sequencing on the next generation sequencing platforms (Roche/454, ABI/SOLiD or Illumina/Solexa).

Has anybody successfully used it for next-gen gene discovery projects?

  • $\begingroup$ This seems to be more like a poll than a question. $\endgroup$ – jonsca May 1 '12 at 11:48
  • 1
    $\begingroup$ Can I turn it into a poll in this website? $\endgroup$ – 719016 May 2 '12 at 7:26
  • $\begingroup$ No, I meant more that poll-type questions can be closed as "not constructive". You should probably reword it a bit to prevent this. $\endgroup$ – jonsca May 2 '12 at 10:02

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