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I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between the rice and the promoter so that I can get good expression levels.

Should I check the expression of the construct in E.coli before transforming it?

What should steps should I follow before finally transforming the construct into rice?

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    $\begingroup$ where do you want to express the protein? $\endgroup$ – WYSIWYG Aug 26 '14 at 4:23
  • $\begingroup$ I will be overexpressing the protein in rice. $\endgroup$ – astroboy89 Aug 26 '14 at 16:45

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