In a microarray, which one is called a target and which one is the probe? the one that is added later , is that the probe or the one present in the slots of the microarray, that is the probe?
(I have not studied biology since last 8 years and now I am going through it because I need it for my research. So if the question seems to silly, forgive me)
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$\begingroup$ The probes are immobilized on the microtitre plate and the labelled targets are hybridized to them. $\endgroup$– canadianerCommented Sep 6, 2014 at 6:25
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1 Answer
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In an RNA microarray, a hybridization event between a probe and target sequence would indicate a presence of the transcript within the sample. The probes are oligonucleotide sequences found on the microarray chip. The target sequences are representative of the entire transcriptome. The targets are found within your biological sample and have gone through many rounds of amplification in addition to labeling with fluorescent dyes. The fluorescently labeled target when bound to the probe can then be detected using a scanning laser.
