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I know that one way is to use the reagent sticks and the refractometer, but this is inaccurate. Is protein distinguishable when using a microscope? Is it time sensitive(i.e. have to test it right away)?

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After some research, I could primarily find two tests for testing the protein content in dog urine.

1) The Urinalysis dipstick test (reference) which has a chance of giving false positive results (reference). Here is the link to a lab test done using the dipstick method and the results that the researchers got.

A sample Dipstick (reference)

2) The second test would be the benzethonium chloride test (reference).

This test precipitates the protein and increases the turbidity of the sample. The turbidity is proportional to the protein concentration. This technique is very sensitive and can yield accurate results in samples with very low protein concentrations (< 20 mg/dL).

A lab study done using this procedure to precipitate protein (reference).

The urine sample is best read within 30 minutes of collection otherwise certain factors (like crystals, bacteria and cells) may alter their composition (dissolve or multiply) over time. If you collect a urine sample at home, please drop it off at your veterinary clinic as soon as possible (reference).

Reasons for excess protein if present in the urine of your pet is listed in this article.

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  • $\begingroup$ Sorry to bother again, did you find out by chance whether the upc ratio test is time sensitive or not? $\endgroup$ – shooting-squirrel Sep 29 '14 at 23:39
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    $\begingroup$ @shooting-squirrel Since the quality of the sample is what could be affected after the aforesaid time period. I believe that the quality of any urine related test after that time period could be skewed. $\endgroup$ – The Last Word Sep 30 '14 at 4:18
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    $\begingroup$ @shooting-squirrel I just found an article which said that if sufficiently refrigerated, the sample should be alright for maybe even 24 hours. Don't worry about it if you handed over the sample to the lab. $\endgroup$ – The Last Word Sep 30 '14 at 6:00

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