In this website, when I want to apply the psi-blast algorithm on a sequence, under the section of algorithm parameters , what does PSI-BLAST threshold mean? The question mark says it means that:
Set the statistical significance threshold to include a sequence in the model used by PSI-BLAST to create the PSSM on the next iteration.
Another parameter is there called pseudocount. Did not understand that too. Could not understand what this means, someone please explain in simple language.