Typically, how many RNA-binding proteins can simultaneously bind to a single mRNA? Or said differently, how many "binding sites" does an mRNA have? What order of magnitude?

I am interested in RNA granules like stress granules or P-bodies. They contain, inter alia, mRNA and RNA-binding proteins. I am not a biologist and I didn't come across this information so far in the related literature.

  • $\begingroup$ In what kind of cells? Bateria or eukaryotic cells? $\endgroup$
    – Chris
    Oct 6 '14 at 8:24
  • $\begingroup$ Both kinds can be of interest to me, as long at is relevant to RNA granules. $\endgroup$
    – David
    Oct 6 '14 at 8:51

See this paper. They have studied RBP-protected sites in the entire human transcriptome by RNA-protein crosslinking followed by RNAse digestion and sequencing: PIPseq.

Figure 1 of the paper shows distribution of protein protected sites in RNAs. They also correlate it with different regions of mRNA and its expression.

They show number of protein protected sites (PPS) per transcript but that is not a proper metric in my opinion. The number should be normalized with transcript length so that you get density of protected sites. From figure 4 (see below) you can roughly estimate that average PPS density is close to 0.6 which means that 60% of any RNA is expected to be protein bound.

                enter image description here

Figure 4

Other points to be noted:

  • Highly translated mRNAs will have multiple ribosomes on their CDS and are likely to be more protected.
  • Sequestered RNAs in stress granules will also have high density of PPS
  • Footprint of different RBPs will be different. So number of proteins that can bind to a mRNA will differ between different RBPs.

Further reading:

  • $\begingroup$ HTH :) You can also have a look at ribosome profiling - sequencing experiments. $\endgroup$
    Oct 6 '14 at 17:15
  • $\begingroup$ @David I added some more links to studies on RBP binding sites $\endgroup$
    Oct 6 '14 at 17:19

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