I've read somewhere that inverted microscope also allows phase contrast microscopy. Is that true? Do all inverted microscope have this ability? Do they have any other functions?

Many thanks in advance.


1 Answer 1


Definitely true, an inverted microscope is one where the optics are positioned below the specimen, and the light source above, providing an optimal situation for looking at a live culture. The Phase rings that shift the light come soon after the light source. For phase, the orientation doesn't matter, just the order:

Upright microscope


(top)eye--->objective--->specimen--->phase--->light source


eye-->Phase compatible objective-->specimen-->phase disk-->light source

Inverted microscope


(top)light source--->phase--->specimen---->Objective--->eye


eye-->phase compatible objective-->specimen-->phase disk-->light source

Not all microscopes have phase no, they are an important and expensive addition to a microscope. Adding phase not only required the phase disk but also a phase compatible objective.

  • $\begingroup$ i tried to start a microscopy SE in Area51, no one gave a @#$% $\endgroup$
    – rhill45
    Oct 16, 2014 at 0:33

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.