Cells "grown in LB broth to an OD600nm"

What does the OD stand for in this?


closed as off-topic by WYSIWYG, Chris, The Last Word, ddiez, biogirl Oct 31 '14 at 6:39

This question appears to be off-topic. The users who voted to close gave this specific reason:

  • "Homework questions are off-topic on Biology unless you have shown your attempt at an answer. For more information see our homework policy." – WYSIWYG, Chris, The Last Word, ddiez, biogirl
If this question can be reworded to fit the rules in the help center, please edit the question.

  • $\begingroup$ Just a google search would have fetched you the answer. $\endgroup$ – WYSIWYG Oct 31 '14 at 5:42
  • $\begingroup$ This phrase in isolation has no meaning, because the value itself is missing: "to OD of X at 600 nm". $\endgroup$ – har-wradim Oct 31 '14 at 16:06

OD = Optical Density

As you grow cells in broth, the broth becomes cloudy. The more cells there are, the cloudier it is. After you grow your cells for a while, you can measure their progress by shining a light through your sample. The more light your cell culture absorbs (higher absorbance is equivalent to higher OD) the more cells there are in it.


Not the answer you're looking for? Browse other questions tagged or ask your own question.