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Cells "grown in LB broth to an OD600nm"

What does the OD stand for in this?

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closed as off-topic by WYSIWYG, Chris, The Last Word, ddiez, biogirl Oct 31 '14 at 6:39

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  • "Homework questions are off-topic on Biology unless you have shown your attempt at an answer. For more information see our homework policy." – WYSIWYG, Chris, The Last Word, ddiez, biogirl
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  • $\begingroup$ Just a google search would have fetched you the answer. $\endgroup$ – WYSIWYG Oct 31 '14 at 5:42
  • $\begingroup$ This phrase in isolation has no meaning, because the value itself is missing: "to OD of X at 600 nm". $\endgroup$ – har-wradim Oct 31 '14 at 16:06
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OD = Optical Density

As you grow cells in broth, the broth becomes cloudy. The more cells there are, the cloudier it is. After you grow your cells for a while, you can measure their progress by shining a light through your sample. The more light your cell culture absorbs (higher absorbance is equivalent to higher OD) the more cells there are in it.

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