I've harvested IPTG induced E.coli BL21DE3 cells' suspension culture by spinning at 5000 rpm/15 min/4 degrees C. The pellet after spin looked pale pink colored. What could be the reasons?
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2$\begingroup$ If you induced them with IPTG what additional genes had you added to induce? $\endgroup$– Samuel BarnettCommented Nov 12, 2014 at 20:18
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$\begingroup$ That would be my question as well: What do you overexpress in the cells? $\endgroup$– Chris ♦Commented Nov 12, 2014 at 20:35
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$\begingroup$ I've seen E. coli colonies with red fluorescent protein that were visibly red even without UV light. That assumes you have cells with RFP. I've heard of carotenoids making bacteria red too, but I don't know what would happen to make E. coli produce them. $\endgroup$– user137Commented Nov 12, 2014 at 21:36
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$\begingroup$ There are a few possibilities why the cells can be pink, so it would be important to know which gene is overexpressed. $\endgroup$– Chris ♦Commented Nov 13, 2014 at 6:54
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