Bovine serum albumin, Avidin, Ficoll-70 and Dextran-70,are they positively charged or negatively charged ? And which other solvents can be used as a substitute to water for preparing solutions in each case ?

  • 2
    $\begingroup$ What part is giving you difficulty. At least for the charges you can use Gooooooooooogle... $\endgroup$
    – nico
    Jun 1 '12 at 6:53
  • $\begingroup$ yes but mainly I require to know about the solvents. $\endgroup$
    – Stp30
    Jun 4 '12 at 4:28
  • $\begingroup$ Flagging because this is a bunch of unrelated questions. (each molecule should be its own question, solvents are also a separate question entirely) The question as is is unlikely to be useful for future users. $\endgroup$
    – Superbest
    May 12 '14 at 18:42

For charge, you could check this one by sequence level, for example for Bovine Albumin (Uniprot: P02769) you can get the theoretical pI of 5.82, as the mean of pKa aminoacid values, to do this can use protparm, so when the pH > pI, the protein has a net negative charge and when the pH < pI, the protein has a net positive charge.

And there are only two organic polar solvents I known to work proteins, DMSO (pKa = 35) and Dimethylformamide (DMF, pKa=-0.01 (20ºC))


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