I am wondering about the logistics of a simple experiment with say 3 types of alcohols, and various concentrations of each, with a control(s)
I would like to research the effects of alcohol on mitosis. Do I need any expensive equipment or is it do-able with standard equipment? I am looking specifically at what I expect to be a negative impact on cell growth. Potential cell death and slowing of mitosis.
"We expect mitosis to slow and stop entirely or become corrupted as the amount of alcohol used increases. Of the three alcohols we will be testing, we expect some to be more damaging than others. The 3 chosen alcohols, listed in order of their hypothesised toxicity are Methanol, Ethanol and 2-methyl-2-butanol."
I am basically trying to see if tertiary alcohols are safer due to ethanols main metabolites acetaldehyde & acetic acid being more harmful/toxic? Since Tertiary alcohols for example, aren't metabolised into aldehydes. Which makes them less toxic than ethanol in theory.
Now I am unsure as to which cells to use to test upon, I'm also unsure as to the legitimacy of my hypothesis. Budget, time and a lack of knowledge is against me. I am researching as much as possible as quick as possible. Thankfully I have help available soon or I would be totally out my depth. This is me out of practice in the field trying to get a head start and help a friend out as best I can. I'll be his assistant and feel thats more than appropriate.
I realise I can't test for too many things. I need one clear goal. If we choose to test certain cells over others do you have any suggestions? and why?
The liver processes ethanol into acetaldehyde & acetic acid. So I would need these chemicals potentially in order to properly test ethanols effects on cells?
I am so out of my depth I feel right now I'm hoping for a prod in the right direction.
The 3 chosen alcohols, listed in order of their hypothesized toxicity are Methanol, Ethanol and 2-methyl-2-butanol. - concentrations of each yet to be considered.
I suffer from dyslexia and my wording is bad at the best of times. But please realize this is a very first draft / ideas on the table. Any help at all is appreciated, apologies for my lack of knowledge in the field. I'm trying to refresh my studies from 10 years ago in 2 days.