I know that there are Nuclear Localisation Sequencenes (NLS). They can be taken from endogenous or viral proteins and fused to the N or C terminus of my recombinant protein.
Which is the best one? Which the best short one (so that it can be more easily added with a Primer)? Should it be attached to the N or the C terminus? Do they influence expression?
I have heard that size does matter (Too large wont fit through the nuclear pore, too smalll will diffuse out again). What is the range of protein sizes that can be targeted efficiently?