In an experiment by Pollard, T.D. and R.R. Weihing, amoeba cells injected with Cytochalasin B were inhibited of movement, while the control group amoeba cells did move. A hypothesis was made that Cytochalasin B blocked polymerization of actin monomers into actin filaments, thus preventing the cell from moving. To validate this hypothesis the experiments were made again with cycloheximide (inhibits new protein synthesis), and dinitrophenol (inhibits new ATP formation), and colchicine (inhibits polymerization of microtubules Here are the results:$\\$
$\begin Condition: % of cells that didn't move No drug 3 Cycochalasin B 95 Colchicine 4 Cycloheximide 3 Cycloheximide + Cycochalasin B 94 Dinitrophenol 5 Dinitrophenol + Cycochalasin B 85$ $\\$
Question: Why were these control important? How did they help validate the hypothesis? Note: this is for self-study.