I wish to find an order of magnitude for the binding/unbinding constants of mRNA and RNA-binding proteins.
Suppose molecules A and B reversibly associate to give the complex C. Assuming the diffusion is large enough such that no concentration gradient exist, the reaction equation at equilibrium is:
𝑘➕[A][B] — 𝑘➖[C] = 0
with 𝑘➕ the binding constant (units: concentration-1.time-1), 𝑘➖ the unbinding constant (units: time-1), and [A], [B], [C] the concentrations of the molecules.
I found many papers assessing the equilibrium constant K = 𝑘+ / 𝑘➖ but I am interested in 𝑘+ and 𝑘➖ independently.