I am working on building a real time PCR machine. Is there any chemistry set that I can purchase online to:
- Identify if PCR amplification was successfully done.
- Test fluorescence dyes that could be used to validate if the fluorescence detector optics worked; i.e. something I could excite with a specific wavelength and monitor for fluorescence.
I am looking for something that is not dangerous and only serves the single purpose of validating the real time PCR machine.
Mods please advise if I need to move to Chemistry StackExchange