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I am interested to culture Wolbachia bacteria in cell line.

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closed as unclear what you're asking by anongoodnurse, Chris, fileunderwater, Luke, dustin Feb 3 '15 at 15:18

Please clarify your specific problem or add additional details to highlight exactly what you need. As it's currently written, it’s hard to tell exactly what you're asking. See the How to Ask page for help clarifying this question. If this question can be reworded to fit the rules in the help center, please edit the question.

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    $\begingroup$ Welcome to Bio.SE! Could your add some details on what kinds of approaches you are thinking? Perhaps some more info on characteristics of Wilbachia? $\endgroup$ – AliceD Feb 2 '15 at 14:21
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    $\begingroup$ If I had to close then I would vote to close this as homework because a simple google search fetches relevant answers. I am not voting to close, however, because Wolbachia is not such a common topic. -1 for lack of effort $\endgroup$ – WYSIWYG Feb 3 '15 at 9:00
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You can see this protocol for Wolbachia isolation:

See this protocol for infecting cell lines with Wolbachia.

In this study the authors have established a cell line from a Wolbachia infected tissue of Aedes albopictus. You can have a look at that too.

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  • $\begingroup$ Why downvote ?? $\endgroup$ – WYSIWYG Feb 3 '15 at 9:45
  • $\begingroup$ I downvoted because I thought it to be somewhat of a link-only answer. Removed. If you add some details I am totally happy to upvote, but likely the question needs work before a proper answer is possible. $\endgroup$ – AliceD Feb 3 '15 at 11:18
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    $\begingroup$ @ChrisStronks Cannot paste the protocol here. Since the OP is asking only for the protocol, I provided the link (all links are to published articles and are not likely to be broken in future). To add anything more, the question needs to be refined $\endgroup$ – WYSIWYG Feb 3 '15 at 12:14
  • $\begingroup$ Agreed, totally. Again, after question refinement I am happy to upvote. $\endgroup$ – AliceD Feb 3 '15 at 12:18

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