Specific genetic engineering of chromosomal aberrations like deletions, inversions and translocations are doable by using the CRISPR/Cas system or the other programmable nuclease systems. Insertions are also possible but mostly limited by the size of the insert.

Here two papers on the topic:



However these methods appear to be inadequate in case you want to insert a long DNA piece (>1Mb) into a chromosome (in case you want to engineer a duplication). As far I have understood, problems arise when 1) you want to make such long DNA in the first place and 2) --suppose you have the DNA-- you need to transfect it into the cell...

Does anyone know how to tackle the genetic engineering of a large chromosomal duplications? Is there any way to induce it without the need of supplementing the extra DNA?


Your Answer

By clicking "Post Your Answer", you acknowledge that you have read our updated terms of service, privacy policy and cookie policy, and that your continued use of the website is subject to these policies.

Browse other questions tagged or ask your own question.