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I have a DNA sequence from a sequencer. How can I determine what protein is it? I tried some translator but it didn't help. What protein is this and how can I determine it?

The sequence: CGTTCTATAGATACGCGATGACGGTATACCATCCGCAAAGT

Thank you very much!

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  • $\begingroup$ Have you tried to blast the sequence? $\endgroup$ – Chris Feb 6 '15 at 17:54
  • $\begingroup$ Translation would fail on this sequence since it’s not a complete open reading frame (doesn’t start with a start codon, doesn’t stop with a stop codon, and length not divisible by three). As such, we don’t even know whether it’s in frame, or maybe shifted by a nucleotide. $\endgroup$ – Konrad Rudolph Feb 7 '15 at 13:41
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    $\begingroup$ Entering fragment in BLASTX helped $\endgroup$ – Serpentes Feb 7 '15 at 14:19
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Enter it in BLASTX. This will give you any protein sequence matches as well as likely homologues given a nucleotide sequence.

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  • $\begingroup$ Great, I hope that was good news for you! $\endgroup$ – James Feb 7 '15 at 15:40
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You might want to try searching against NR/NT database using BLAST. This way you will get to know what this sequence might be similar too. The length of the sequence is too short to code for any meaningful protein.

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