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The colorimetric assays by Biovision and Sigma Aldrich seem to utilise a probe that binds to or reacts with NADH in order to cause absorbance at 450nm which can then be quantified by a spectrophotometer.

What is the chemical basis of this reaction?

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    $\begingroup$ I don't think they would reveal it but perhaps the substrate is this molecule. $\endgroup$ – WYSIWYG Mar 2 '15 at 17:09
  • $\begingroup$ @WYSIWYG Thanks! You should convert that to an answer. $\endgroup$ – March Ho Mar 2 '15 at 17:25
  • $\begingroup$ I am not sure if that is the same compound. $\endgroup$ – WYSIWYG Mar 2 '15 at 17:33
  • $\begingroup$ They will most likely not reveal which substance they are using. Most likely it is something which is oxidized by NADH and then gives a blue color. $\endgroup$ – Chris Mar 2 '15 at 21:54
  • $\begingroup$ @Chris it absorbs 450nm it may emit a longer wavelength. $\endgroup$ – WYSIWYG Mar 3 '15 at 8:21

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