3
$\begingroup$

I already know about recombinases (specifically excisionases), but was wondering if there were other mechanisms present.

$\endgroup$
  • $\begingroup$ Welcome to Biology S.E.! If you need additional assistance, please visit The Help Center. $\endgroup$ – L.B. Mar 4 '15 at 0:23
  • $\begingroup$ The absolute standard way has to be PCR. en.wikipedia.org/wiki/Polymerase_chain_reaction Design primers for each end of a known sequence, provide DNA containing that sequence, and (hey presto!) amplify the length between the primers. $\endgroup$ – Teige Mar 4 '15 at 11:33
  • $\begingroup$ If you're asking about how sequences are excised in nature, an interesting case is the CRISPR-Cas viral defence system of bacteria, where sequences adjacent to a particular "handle" or "signal" in the viral genome are excised and added to an array in the bacterial genome which acts as a memory for immunity. I don't know how the mechanism works! $\endgroup$ – Teige Mar 4 '15 at 11:40
  • $\begingroup$ What about endonucleases? $\endgroup$ – rhill45 Mar 4 '15 at 15:43
  • $\begingroup$ I know about the Cas9 mechanism as well. I guess my question was more oriented towards the "cutting out" of a sequence of DNA and then a religation of the two flanking ends. Cas9 does the first part, but not the second part, while recombinases do both. $\endgroup$ – The Obscure Question Mar 5 '15 at 20:00
1
$\begingroup$

Genome editing techniques like CRISPR-Cas and TALEN use non-specific recombinases coupled to a DNA-recognizing part that can be designed to be specific for any stretch of sequence, as it is constructed out of single zinc-finger 'modules' that each specifically bind a couple of nucleotides (one nucleotide each in the case of TALEN).

$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.