Antarctic Phosphatase is used to catalyze the removal of 5' phosphate from DNA. If I'm using it in the lab on a sample of 1-5 micrograms of digested DNA, how would I figure out how long to incubate the sample at 37 degrees Celsius?
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For 5' extensions or blunt ends, incubate the sample for 15 minutes at 37 degrees Celsius.
For 3' extensions, incubate the sample for an hour.