Not all the female cheek cells show Barr Bodies when stained with Giemsa stain? Only 30 to 40% female cells show Barr Bodies. Why?
We washed mouth twice with listerine to reduce bacteria count, then scraped cheek cells with moistened ear bud then smeared that on clean glass slide. then air drying the smear we fixated it with methanol, then after air drying we applied thin layer of giemsa stain and after 15 minutes drained and washed off extra stain. the cells were stained and we could see the nucleus but nobody could see barr bodies. it was an experiment in our undergraduate lab and nobody could identify barr bodies in female cells. very few samples had barr bodies.