I am trying to get a good pair (or better yet, library of pairs) of primers that give me one band in WT mice (C57BL/6). Do you know where I could find such a thing?
Alternatively, I thought of just designing a few sets of primers myself for highly-conserved regions. But that's quite a bit of guessing work (what regions, what pairs from what
eprimer3 will calculate for me etc.).
- Do you know where such a database could be found?
- If not, could you help me get started with creating it myself? What specific regions would in your opinion be particularly good for this purpose?