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How do I assess the quality of a cDNA library?

I want to clone CDS copies of genes from a library, but I don't know what's a typical expectation of getting a full length clone even for a shorter gene (~1000 bp). I know it doesn't have to be composed of full length cDNA for sequencing, but I'd imagine quality of starting materials is important there too.

Are there some common transcripts I can try to obtain from PCR that will tell me how fragmented the sequences are in there?

What's a typical result from a commercial cDNA library - are there any QC tests that are quick to run in the lab?

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  • $\begingroup$ From what kind of cells do you have your library? I think the answer heavily depends on that info. $\endgroup$ – Nandor Poka Apr 23 '15 at 13:24
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    $\begingroup$ did you try googling? actin seems to be useful probe target: ncbi.nlm.nih.gov/pubmed/3273856 $\endgroup$ – aaaaaa Apr 23 '15 at 16:43
  • $\begingroup$ @poka.nandor interesting - human testis or spleen - does that help? $\endgroup$ – shigeta Apr 23 '15 at 18:49
  • $\begingroup$ quite a lot! At least we know what genes should be expressed, however there are housekeeping genes that expressed in most or all cell types like actin as aandreev suggested. $\endgroup$ – Nandor Poka Apr 23 '15 at 18:52
  • $\begingroup$ Is this library for sequencing? What is your experimental setup? Did you perform reverse transcription and you want to check how well it went? I deleted my answer as when I tried to work on it it raised more questions on the post. $\endgroup$ – Nandor Poka Apr 23 '15 at 19:17

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