How do I assess the quality of a cDNA library?
I want to clone CDS copies of genes from a library, but I don't know what's a typical expectation of getting a full length clone even for a shorter gene (~1000 bp). I know it doesn't have to be composed of full length cDNA for sequencing, but I'd imagine quality of starting materials is important there too.
Are there some common transcripts I can try to obtain from PCR that will tell me how fragmented the sequences are in there?
What's a typical result from a commercial cDNA library - are there any QC tests that are quick to run in the lab?