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Reading this study http://www.nature.com/cr/journal/v23/n10/full/cr2013122a.html

They writing

Multiple exogenous and endogenous genes can be simultaneously activated by CRISPR-on

We tested single, double and triple activation of a TetO::tdTomato transgene and the endogenous SOX2 and IL1RN genes (Figure 3A) in HEK293T cells carrying the stably integrated TetO::tdTomato transgene

Could you please help me to understand: They talking about exogenous gene. But saying "carrying the stably integrated". Does that mean that this gene (TetO::tdTomato) is in the cell genome (integrated to genome)? If so, what is the meaning of exogenous here?

Thank you!

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According to Merriam Webster, an exogeneous gene is defined as " introduced from or produced outside the organism or system; specifically : not synthesized within the organism or system—compare endogenous".

I believe they refer to the tetO::tdTomato transgene as an exogeneous, or not originating from the species of study

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  • $\begingroup$ Yes thats what I am understanding from this too. Thank you. $\endgroup$ – Robertos Apr 26 '15 at 16:22
  • $\begingroup$ @Barbos if this answered your question, please consider accepting it by clicking on the check mark to turn it green. $\endgroup$ – MattDMo Apr 26 '15 at 21:07

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