I need to stop some protein from being active and searching for some universal way to do so. In mammalians.
With CRISPR it is possible to knock-out the entire gene. But it's a little complicate (need two gRNA for eg.). There are also another reasons, so I need to do this with only one gRNA.
So I am thinking about inserting some mutation.
My question: Is there way to make some mutation that will prevent creation of functional protein, with high efficiently - with one gRNA. And without need to know the active site of protein.
For example, frame-shift or mutation around start codon? Is it possible? If so, how can I do this? To where I need to send the gRNA?