First of all I have to say that I have no biology background since I'm a undergraduate computer science student. Nowadays, for my research I need to use some of the databases related with bioinformatics (modENCODE
and NCBI-GEO
). Currently, I'm searching RNA-Seq data and I have found the following one in modENCODE website which is stored in NCBI-GEO database.RNA-Seq data
In the description part of it, it is written that:
Synchronized L1 worms cultured at 25degC for about 91 hrs to a point at which ~100% worms are resistant to 1% SDS treatment. Illumina sequencing of C. elegans dauer daf-2(el370) sample 2-1 DauerDAF2-2-1 polyA+ RNAseq random fragment library This sample is available through the modENCODE DCC (www.modencode.org) as: DauerDAF2-2-1 Local library name: DauerDAF2-2-1 Quality values are ASCII encoded Phred + 33.
Regarding with this paragraph, I want to ask that, what is C. elegans dauer daf-2(el370)
. I've searched on the internet and I have found that dauer
is kind of development stage of C. elegans. However, I could not find any information about the daf-2(el370)
part (Actually all I found was that daf-2 is name of a gene of C. elegans). Actually, my primarily concern is whether this RNA-Seq data contains the C. elegans' whole transcriptome or it contains only mRNAs related with daf-2 gene.