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In the context of the bacterial systems (not the gene editing tool), I was wondering what happens to the foreign DNA after the Cas proteins have created a new spacer.

It is really not clear to me, most of the documentation I have found focuses on the subsequent steps (expression and interference) and do not discuss the fate of said foreign DNA. For example, see this image from wikipedia https://en.wikipedia.org/wiki/CRISPR#/media/File:Crispr.png

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  • $\begingroup$ It's not really clear to me what you mean. Do you mean what happens to the foreign DNA or what happens to the spacer? The former is cleaved to form a spacer, the latter is inserted into the genome. $\endgroup$ – Amory Jun 1 '15 at 21:00
  • $\begingroup$ Ok thanks, how do you know that it is cleaved? Are there endonuclease activities in the proteins that deal with the foreign DNA? I was under the impression that the spacer was created by copy of the protospacer of interest. $\endgroup$ – AlterSid Jun 1 '15 at 21:06
  • $\begingroup$ @AlterSid Cas is that endonuclease. Please read about CRISPR-Cas and ask specific questions if you have any. Wikipedia is good enough. $\endgroup$ – WYSIWYG Jun 2 '15 at 4:29
  • $\begingroup$ Try this paper ncbi.nlm.nih.gov/pubmed/20056882 it makes a nice overview of the all process and if you want to go deeper check the references, they cover all the passages involved in the CRISPR mediated immunity. $\endgroup$ – alec_djinn Jun 2 '15 at 16:17

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