Three polymorph microsatellite markers are used to try and narrow down the location of a disease locus, with the use of PCR with 2 flanks on each side of the actual polymorphic area. The PCR product is then put on gel.
I need help understanding exactly what is going on here. I understand polymorph as "two or more phenotypes in the same population" as by wikipedia. I don't understand what they mean here.
How is the PCR different with 2 flanks from a normal PCR?
I do understand how the gel works.