I am looking for a cell line (preferably hematopoietic) that has an inactivating DNMT3a mutation. I have checked the Cancer Cell Line Encyclopedia but of all the hematopoietic cell lines I know I can't find any with a DNMT3a mutation.

It would be great if somebody knows of a cell line with a DNMT3a mutation, but it would also be helpful if somebody knows of a database that I could use to find this information in the future. Thanks.

  • $\begingroup$ Why not just induce one? There are quite easy-to-use CRISPR/Cas kits available nowadays. $\endgroup$ – MattDMo Jun 19 '15 at 23:36
  • $\begingroup$ While that would work, it would be much cheaper and easier to identify which of the cell lines at my disposal contain such a mutation. $\endgroup$ – The Nightman Jun 19 '15 at 23:40
  • $\begingroup$ All I'm saying is if you can't find a cell line that meets your needs, it would be quite cost-effective to generate one on your own. There are some extra costs outside of the kit, but this Life Technologies kit is only US$514, whereas purchasing a new cell line from ATCC not in your collection may cost an approximately equivalent amount, depending on the line, what kind of institution you're at, etc. Worth thinking about. $\endgroup$ – MattDMo Jun 19 '15 at 23:51
  • $\begingroup$ For CRISPR-Cas you don't even need the kit. Just have a plasmid for containing the Cas9 gene from where you can IVT the Cas9 mRNA. Co-transfect this with the donor plasmid. $\endgroup$ – WYSIWYG Jun 20 '15 at 11:46
  • $\begingroup$ Or for only $60 from AddGene $\endgroup$ – Luigi Jun 20 '15 at 18:12

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