this is my first question on BiologySE. I am a Physics and Mathematics student currently doing a project on cell growth simulation. I am doing literature survey and I have a question about cellular biology experimental methods.
I understand that the membrane potential is a measure of mitochondrial functionality (higher potential means more efficient electron transport chain). Additionally, the mitochondrial volume is also an indicator of mitochondrial efficiency (higher volume, higher area, more places to do electron transport).
I would like to have some points elucidated.
In particular, can these mitochondrial membrane potential and volume...
be measured for a single cell, or only in a population?
be measured non-destructively, leaving the cell alive?
be measured simultaneously, or experiments measuring one necessarily make the other unavailable?
I am thinking mostly of in vitro experiments of course.