I've a question about ORF and translation into protein. Say I have an RNA transcript that contains two ORFs, one in phase 1 ; one in phase2 ; as:
ORF number 1 in reading frame 1 on the direct strand extends from base 529 to base 759.
ORF number 2 in reading frame 3 on the direct strand extends from base 849 to base 1223.
So ORF number 1 comes earlier in the transcript, but ORF number 3 is longer ..
Is it always the first ORF that is translated ?
It's in an eukaryoytic organism and same gene (it's the same transcript)
I use ORF finder (http://www.bioinformatics.org/sms2/orf_find.html) on my transcript sequence and it gives me two potential ORFs (ORF 1 and ORF 2 within two different frames)