6
$\begingroup$

Are some non-coding RNA spliced like mRNA? I tried to find some information but I don't find anything...

Improve this question
$\endgroup$
1
  • $\begingroup$ yes yes - many of them are spliced out of mRNA too $\endgroup$
    – shigeta
    Sep 19, 2012 at 16:23

3 Answers 3

Reset to default
9
$\begingroup$

You should check out Howald C, et al[1]. This is one of the many recent papers tied to the ENCODE data. They've used RT-PCR to amplify exon-exon junctions and then sequenced the results. Supplemental table 2 shows 3076 validated exon-exon junctions in putative processed transcripts which, in the main body of the paper may be sub-classified as:

Non-coding, 3prime_overlapping_ncrna, Ambiguous_orf, Antisense, LincRNA, ncRNA_host, Retained_intron, Sense_intronic, Sense_overlapping, Processed transcript.

  1. Howald C, Tanzer A, Chrast J, Kokocinski F, Derrien T, Walters N, Gonzalez JM, Frankish A, Aken BL, Hourlier T, Vogel JH, White S, Searle S, Harrow J, Hubbard TJ, Guigó R, Reymond A. 2012. Combining RT-PCR-seq and RNA-seq to catalog all genic elements encoded in the human genome. Genome Research, 22: 1698–1710, doi:10.1101/gr.134478.111.
Improve this answer
$\endgroup$
4
$\begingroup$

Do you call ribosomal RNA non-coding? rRNA is spliced both in prokaryotes and eukaryotes.

Improve this answer
$\endgroup$
4
  • 1
    $\begingroup$ can you give some reference, please? $\endgroup$
    – R Stephan
    Sep 19, 2012 at 14:08
  • $\begingroup$ Sure. Some general information on pre-tRNA and pre-rRNA processing from Molecular Cell Biology: ncbi.nlm.nih.gov/books/NBK21729 $\endgroup$
    – jkadlubowska
    Sep 19, 2012 at 17:02
  • $\begingroup$ And of course rRNA is non-coding. Coding refers specifically to sequences coding for protein. $\endgroup$
    – terdon
    Sep 21, 2012 at 11:27
  • $\begingroup$ One of my teachers considered all "functional" RNA coding. $\endgroup$
    – jkadlubowska
    Sep 21, 2012 at 18:20
4
$\begingroup$

Yes, check out HOTAIR (in human), as well as cyrano and megamind in zebrafish -- they are all spliced.

Improve this answer
$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.