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I've received freeze-dried bacteria that I intend to use for a study I'm doing on slime mold; however, I've never dealt with freeze-dried, only frozen, bacterial cells. After reconstituting these cells in 6mL media, is it alright to split this in to halves and place one aliquot into DMSO or glycerol to make a permanent stock right away? Or is it best to plate, isolate, suspend, grow, and then make a permanent stock?

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  • $\begingroup$ I was under the impression that glycerol stocks should be made from log phase cells. Therefore, I would definitely grow up the cells before freezing a glycerol stock. $\endgroup$ – March Ho Jul 22 '15 at 2:24
  • $\begingroup$ I am not sure, if the cells would survive this procedure. I would reconstitute them first and then make glycerol or DMSO (which I prefer for easier handling) from fresh cultures. $\endgroup$ – Chris Jul 22 '15 at 6:30

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