I'm trying to empirically calculate the approx final magnification (mag) of digital images rendered through our confocal microscope. Using a micrometer and the microscopy equipment I have, the following is known:
Objective mag = 63x
Ocular mag =10x
Diameter of field of view when viewing specimen through occular = 320µm
Diameter of field of view when live viewing specimen on the screen = 100µm
What is the final mag of the rendered image on the screen?
This is not a homework question. The manufacturers specification for the degree of magnification that the camera and confocal (and obviously there is some) provide is unknown.