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Searching for some protein, that I could use as extracellular marker for mammalian cells. I need to insert mRNA to cell, and to detect the protein outside the cell (not on membrane) if the mRNA successfully enter and been translated. (not GFP, I need protein that exit from the cell in big numbers)

Thank you.

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    $\begingroup$ You might want to check secretome analyses like nature.com/nbt/journal/v30/n10/full/nbt.2356.html $\endgroup$ – 243 Aug 2 '15 at 10:11
  • $\begingroup$ I don't know of any that are exported by all cells, e.g. both neurons and . All blood proteins, e.g. serum albumin are exported but by specific cells. $\endgroup$ – Gustav CFC Aug 2 '15 at 16:57
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You can always express GFP or your favorite protein in a secretory vector like pSecTag or pSecTag2 from Invitrogen/Life/ThermoFisher/SuperUltraBioMegaMart. There are probably others, but those were the first ones I came across. They contain the mouse Igκ signal sequence for efficient secretion (so the website says, I've never used it).

I don't know the details of your experiment, but are you sure you want to use a secreted protein as a transfection marker? Remember, it's possible that only a small fraction of your cell population will take up the vector (or mRNA, if that's what you're using, but there are issues with that), but you will see secreted protein regardless of the number of cells that take it up.

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  • $\begingroup$ Thank you! Looks good. It's ok, I just need to know if some cells get the vector, doesn't meter how many of them. $\endgroup$ – Robertos Aug 3 '15 at 8:05
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SEAP, secreted alkaline phosphatase, and vectors engineered to express fusions to this reporter gene may be well-suited to solve your problem.

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