First post here, so bare with me if I violate any etiquette or formatting rules.
Let's say I've got a protein in humans. When it interacts with an ncRNA found in humans, it does something that I'm very interested in. I see a highly homologous (~85% AA identity) protein in plants, and it's widely conserved. I'm interested in eliciting the same activity of this protein in plants. Only problem is that there's no native plant-produced ncRNA to interact with my protein of interest in the same way. So I had an idea:
What if I introduce a transcript to plants that addresses this problem? If the plant version of this protein interacts with this (introduced) RNA in the same way it will be really cool. I'd like to test this hypothesis with some synthesized constructs. But before I plunk down thousands of dollars on a few constructs, I'd like to explore any predictive or analytical tools to strengthen the case. I'm aware the odds are against me.