How much penicillinase is needed to deactivate 125mg of amoxicillin?
Penicillinase in penase concentrate from CPC. 20,000 Levy Units/ml/min
I need IU of 125 mg of amoxicillin.
Please note that the problem is with finding the right parameters for amoxicillin, not penicillin G.
My goal is to test an amoxicillin product (i.e. it contains more than just amoxicillin) so the overall problem is more complex, I wanted to keep the question focused.
I would like to know the minimal amount of penase needed to deactivate 125mg of amoxicillin (that's the amount of amoxicillin in the product) in a reasonable amount of time, say 5 minutes. I want the minimal amount of penase because I am not sure what is its influence on bacterial growth, I am going to test that separately. Maybe I am going wrong about the whole problem?
I think that this paper could be helpful in general: Microbiological Applications of the Inactivation of Antibiotics and Other Antimicrobial Agents but it's behind a paywall and I can't access it at the moment. So far, I failed to find any other useful resources.